Effect of a combination of TGFβ and IGF growth factors on the chondrogenic potential of mesenchymal bone marrow stem cells
https://doi.org/10.29235/1818-9857-2021-2-78-83
Abstract
The authors studied the influence of the combination of TGFβ and IGF growth factors, as well as the differentiation time, on the induction of MSC chondrogenesis in vitro. It is proved that MSCs located in 2D and 3D systems, when exposed to TGFβ/ IGF, showed the signs of early chondroblast- like cells in 7 days. The TGFβ/ IGF used for the induction of MSCs is more preferred, because it results in a more pronounced hypertrophic-suppression effect. The absence of significant differences in gene expression (excepting Sox9) on the 7th and 21st days of chondrogenic differentiation allows the process to be reduced in vitro to 7 days.
About the Authors
A. Zhernosechenko
Республиканский научно-практический центр детской онкологии, гематологии и иммунологии
Belarus
Belarus
Y. Isaykina
Республиканский научно-практический центр детской онкологии, гематологии и иммунологии
Belarus
Belarus
T. Filipovich
Республиканский научно-практический центр детской онкологии, гематологии и иммунологии
Belarus
Belarus
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Review
For citations:
Zhernosechenko A., Isaykina Y., Filipovich T. Effect of a combination of TGFβ and IGF growth factors on the chondrogenic potential of mesenchymal bone marrow stem cells. Science and Innovations. 2021;(2):78-83. (In Russ.) https://doi.org/10.29235/1818-9857-2021-2-78-83
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