innosferaНаука и инновацииScience and Innovations1818-98572412-9372Издательский дом «Белорусская наука»10.29235/1818-9857-2024-05-79-83innosfera-619Research ArticleДИССЕРТАЦИОННЫЕ ИССЛЕДОВАНИЯDISSERTATION RESEARCHПартанатоз при вирус-ассоциированном раке печени и толстого кишечникаParthanatosis in virus-associated liver and colon cancerКузнецовО.KuznetsovO.

Олег Кузнецов, директор

Минск

Oleg Kuznetsov, Director

Minsk

olegkuznetsov@inbox.ruЦыркуновВ.TsirkunovV.

Владимир Цыркунов, профессор, профессор кафедры

кафедра инфекционных болезней

Гродно

Vladimir Tsirkunov, Professor, Professor of the Department

Department of Infectious Diseases

Grodno

tvm111@mail.ruИнститут биохимии биологически активных соединений НАН БеларусиInstitute of Biochemistry of Biologically Active Compounds NAS of BelarusГродненский государственный медицинский университетGrodno State Medical University202413062024057983Copyright © Издательский дом «Белорусская наука», 20242024Издательский дом «Белорусская наука»Издательский дом «Белорусская наука»https://innosfera.belnauka.by/jour/about/submissions#copyrightNoticehttps://innosfera.belnauka.by/jour/article/view/619

Рассмотрен биологический маркер партанатоза – Poly (ADP-ribose) polymerase (PARP), активация которого связана с патологическими процессами в организме. Исследованы образцы тканей опухолей у пациентов с морфологически верифицированным раком печени и раком толстого кишечника. Контролем выступали ткани данных органов, не пораженные опухолью, и аналогичные прижизненные биоптаты (нативная ткань) неонкологических больных. Оценка концентрации антител к PARP и определение ДНК/РНК вирусов (VEB, HBV, HCV, HPV, CMV, HSV1/2, HHV6) в указанных образцах проведены методами иммуноферментного анализа и полимеразной цепной реакции. Результаты исследований показали, что концентрация антител к PARP в экстрактах ткани при опухолевых процессах выше, чем в нативных образцах (p<0,05) и в ткани с отсутствием критериев злокачественности (p<0,05). Зависимость между частотой выделения из тканей опухолей ДНК-содержащих вирусов (HHV6, CMV, VEB) и концентрацией антител к PARP (p<0,05) позволяет утверждать о стимулировании повреждения ДНК клеток под действием вирусов при онкогенезе печени и толстого кишечника.

The biological marker of parthanatosis is Poly (ADP-ribose) polymerase (PARP), the activation of which is associated with various pathological processes in the body. Tumor tissue samples from patients with morphologically verified liver and colon cancer were studied. The control consisted of liver and intestinal tissues of the same patients not affected by a tumor, and intravital biopsies (native tissue) of the liver and intestines of non-cancer patients. Determination of the concentration of antibodies to PARP and determination of DNA/RNA viruses (VEB, HBV, HCV, HPV, CMV, HSV1/2, HHV6) in tissue samples was carried out by enzyme immunoassay and polymerase chain reaction method. The research results showed that the concentration of antibodies to PARP in tissue extracts from tumor processes is higher than in native tissue samples (p<0.05) and in tissue with «no criteria for malignancy» (p<0,05). The relationship between the frequency of isolation of DNA-containing viruses (HHV6, CMV, VEB) from tumor tissues and the concentration of PARP antibodies (p<0,05) suggests that DNA damage to cells is stimulated by viruses during oncogenesis of the liver and colon.

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The authors declare that there are no conflicts of interest present.