innosferaНаука и инновацииScience and Innovations1818-98572412-9372Издательский дом «Белорусская наука»10.29235/1818-9857-2021-2-78-83innosfera-190Research ArticleДИССЕРТАЦИОННЫЕ ИССЛЕДОВАНИЯDISSERTATION RESEARCHВлияние комбинации ростовых факторов TGFβ и IGF на хондрогенный потенциал мезенхимальных стволовых клеток костного мозгаEffect of a combination of TGFβ and IGF growth factors on the chondrogenic potential of mesenchymal bone marrow stem cellsЖерносеченкоА.ZhernosechenkoA.

научный сотрудник лаборатории клеточных биотехнологий и цитотерапии, гематологии и иммунологии

sapphire.anna@gmail.comИсайкинаЯ.IsaykinaY.

завлабораторией клеточных биотехнологий и цитотерапии, кандидат биологических наук

yaninai@mail.ruФилиповичТ.FilipovichT.

младший научный сотрудник

tatianafilipovich95@gmail.comРеспубликанский научно-практический центр детской онкологии, гематологии и иммунологииBelarus202118122022027883Copyright © Издательский дом «Белорусская наука», 20222022Издательский дом «Белорусская наука»Издательский дом «Белорусская наука»https://innosfera.belnauka.by/jour/about/submissions#copyrightNoticehttps://innosfera.belnauka.by/jour/article/view/190

Исследовано влияние комбинации ростовых факторов TGFβ (TGFβ1 и TGFβ3) и IGF, а также срока дифференцировки на индукцию хондрогенеза МСК in vitro. Доказано, что МСК, находящиеся в 2D и 3D‑системах, при воздействии TGFβ/IGF через 7 суток имели признаки ранних хондробластоподобных клеток. Применение TGFβ3/IGF для хондродифференцировки МСК предпочтительнее, так как приводит к более выраженному ингибированию гипертрофии клеток. Отсутствие достоверных различий в экспрессии генов (за исключением Sox9) на 7-е и 21-е сутки хондродифференцировки МСК позволяет сократить продолжительность процесса до 7 суток.

The authors studied the influence of the combination of TGFβ and IGF growth factors, as well as the differentiation time, on the induction of MSC chondrogenesis in vitro. It is proved that MSCs located in 2D and 3D systems, when exposed to TGFβ/ IGF, showed the signs of early chondroblast- like cells in 7 days. The TGFβ/ IGF used for the induction of MSCs is more preferred, because it results in a more pronounced hypertrophic-suppression effect. The absence of significant differences in gene expression (excepting Sox9) on the 7th and 21st days of chondrogenic differentiation allows the process to be reduced in vitro to 7 days.

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The authors declare that there are no conflicts of interest present.