<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">innosfera</journal-id><journal-title-group><journal-title xml:lang="ru">Наука и инновации</journal-title><trans-title-group xml:lang="en"><trans-title>Science and Innovations</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">1818-9857</issn><issn pub-type="epub">2412-9372</issn><publisher><publisher-name>Издательский дом «Белорусская наука»</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.29235/1818-9857-2022-4-71-78</article-id><article-id custom-type="elpub" pub-id-type="custom">innosfera-17</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ДИССЕРТАЦИОННЫЕ ИССЛЕДОВАНИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>DISSERTATION RESEARCH</subject></subj-group></article-categories><title-group><article-title>Визуализирующая проточная цитометрия для медико-биологических исследований</article-title><trans-title-group xml:lang="en"><trans-title>Imaging flow cytometry for life sciences</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Гончаров</surname><given-names>А.</given-names></name><name name-style="western" xml:lang="en"><surname>Goncharov</surname><given-names>A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Андрей Гончаров, директор, кандидат медицинских наук, доцент</p><p> </p></bio><bio xml:lang="en"><p>Andrey Goncharov </p></bio><email xlink:type="simple">andrei.hancharou@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff xml:lang="ru" id="aff-1"><institution>Институт биофизики и клеточной инженерии НАН Беларуси</institution><country>Belarus</country></aff><pub-date pub-type="collection"><year>2022</year></pub-date><pub-date pub-type="epub"><day>20</day><month>04</month><year>2022</year></pub-date><volume>0</volume><issue>4</issue><fpage>71</fpage><lpage>78</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Издательский дом «Белорусская наука», 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Издательский дом «Белорусская наука»</copyright-holder><copyright-holder xml:lang="en">Издательский дом «Белорусская наука»</copyright-holder><license xlink:href="https://innosfera.belnauka.by/jour/about/submissions#copyrightNotice" xlink:type="simple"><license-p>https://innosfera.belnauka.by/jour/about/submissions#copyrightNotice</license-p></license></permissions><self-uri xlink:href="https://innosfera.belnauka.by/jour/article/view/17">https://innosfera.belnauka.by/jour/article/view/17</self-uri><abstract><p>Представлены основные сведения о визуализирующей проточной цитометрии (ВПЦ), ее отличия от традиционной цитофлуориметрии и флуоресцентной микроскопии. Изложены принципы данного метода, его возможности в анализе клеток, микроорганизмов и микрочастиц, области применения, описано устройство приборов, процессы учета и анализа данных.</p></abstract><trans-abstract xml:lang="en"><p>The review presents the basic information about the method of imaging flow cytometry and the main differences from traditional flow cytometry and fluorescence microscopy. The principles of the method, the design of instruments, the process of data collection and analysis are described in detail. The fields of application of the method and its possibilities in the analysis of cells, microorganisms and microparticles are described. </p><p> </p></trans-abstract><kwd-group xml:lang="ru"><kwd>визуализирующая проточная цитометрия</kwd><kwd>проточная цитометрия</kwd><kwd>иммунология</kwd><kwd>клеточная биология</kwd><kwd>флуоресценция</kwd></kwd-group><kwd-group xml:lang="en"><kwd>flow cytometry</kwd><kwd>imaging flow cytometry</kwd><kwd>immunology</kwd><kwd>cell biology</kwd><kwd>fluorescence</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Manohar S.M. Flow cytometry: principles, applications and recent advances / S.M. Manohar [et al.] // Bioanalysis. 2021. Vol. 13, №3. P.181–198.</mixed-citation><mixed-citation xml:lang="en">Manohar S.M. Flow cytometry: principles, applications and recent advances / S.M. Manohar [et al.] // Bioanalysis. 2021. Vol. 13, №3. P.181–198.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">McKinnon K.M. Flow Cytometry: An Overview / K.M. McKinnon // Curr Protoc Immunol. 2018. Ch. 5.1.1–5.1.11.</mixed-citation><mixed-citation xml:lang="en">McKinnon K.M. Flow Cytometry: An Overview / K.M. McKinnon // Curr Protoc Immunol. 2018. Ch. 5.1.1–5.1.11.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Chelkar M. Flow cytometry: Principle and applications / M. Chelkar, S.Panda// Pharma Innov. J. 2020. Vol. 9, №10. P.6–9.</mixed-citation><mixed-citation xml:lang="en">Chelkar M. Flow cytometry: Principle and applications / M. Chelkar, S.Panda// Pharma Innov. J. 2020. Vol. 9, №10. P.6–9.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">BD Spectrum Viewer // BD Biosciences. https://www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer.</mixed-citation><mixed-citation xml:lang="en">BD Spectrum Viewer // BD Biosciences. https://www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Amnis® ImageStream®X Mk II // Luminex Corp. https://www.luminexcorp.com/imagestreamx-mk-ii.</mixed-citation><mixed-citation xml:lang="en">Amnis® ImageStream®X Mk II // Luminex Corp. https://www.luminexcorp.com/imagestreamx-mk-ii.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Doan M. Diagnostic Potential of Imaging Flow Cytometry/ M. Doan [et al.] // Trends Biotechnol. 2018. Vol. 36, №7. P.649–652.</mixed-citation><mixed-citation xml:lang="en">Doan M. Diagnostic Potential of Imaging Flow Cytometry/ M. Doan [et al.] // Trends Biotechnol. 2018. Vol. 36, №7. P.649–652.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Lippeveld M.Classification of Human White Blood Cells Using Machine Learning for Stain-Free Imaging Flow Cytometry/ Maxim Lippeveld[et al.] // Cytometry Part A. 2020. Vol. 97, №3. P.308–319.</mixed-citation><mixed-citation xml:lang="en">Lippeveld M.Classification of Human White Blood Cells Using Machine Learning for Stain-Free Imaging Flow Cytometry/ Maxim Lippeveld[et al.] // Cytometry Part A. 2020. Vol. 97, №3. P.308–319.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Bargieri D.Y. Apical membrane antigen 1 mediates apicomplexan parasite attachment but is dispensable for host cell invasion / D.Y. Bargieri [et al.] // Nature Communications. 2013. Vol. 4. P.2552.</mixed-citation><mixed-citation xml:lang="en">Bargieri D.Y. Apical membrane antigen 1 mediates apicomplexan parasite attachment but is dispensable for host cell invasion / D.Y. Bargieri [et al.] // Nature Communications. 2013. Vol. 4. P.2552.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Barteneva N . S . Circulating microparticles: Square the circle / N.S. Barteneva[et al] // BMC cell biology. 2013. Vol. 14, №23. P.21.</mixed-citation><mixed-citation xml:lang="en">Barteneva N . S . Circulating microparticles: Square the circle / N.S. Barteneva[et al] // BMC cell biology. 2013. Vol. 14, №23. P.21.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Baruch K.Cerebral nitric oxide represses choroid plexus NFκB-dependent gateway activity for leukocyte trafficking/ K. Baruch [et al.] // The EMBO Journal. 2015. Vol. 34, №13. P.1816–1828.</mixed-citation><mixed-citation xml:lang="en">Baruch K.Cerebral nitric oxide represses choroid plexus NFκB-dependent gateway activity for leukocyte trafficking/ K. Baruch [et al.] // The EMBO Journal. 2015. Vol. 34, №13. P.1816–1828.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Begum J. A method for evaluating the use of fluorescent dyes to track proliferation in cell lines by dye dilution / J. Begum[et al.] // Cytometry. Part A: the journal of the International Society for Analytical Cytology. 2013. Vol. 83, №12. P.1085–1095.</mixed-citation><mixed-citation xml:lang="en">Begum J. A method for evaluating the use of fluorescent dyes to track proliferation in cell lines by dye dilution / J. Begum[et al.] // Cytometry. Part A: the journal of the International Society for Analytical Cytology. 2013. Vol. 83, №12. P.1085–1095.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Chang S. Impaired expression and function of toll-like receptor 7 in hepatitis C virus infection in human hepatoma cells / S.Chang, K. Kodys, G.Szabo// Hepatology. 2010. Vol. 51, №1. P.35–42.</mixed-citation><mixed-citation xml:lang="en">Chang S. Impaired expression and function of toll-like receptor 7 in hepatitis C virus infection in human hepatoma cells / S.Chang, K. Kodys, G.Szabo// Hepatology. 2010. Vol. 51, №1. P.35–42.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">ChoyC.H. Aggregation and size attributes analysis of unadsorbed and adjuvantadsorbed antigens using a multispectral imaging flow cytometer platform / C.H.Choy[et al] // Journal of pharmaceutical sciences. 2021.</mixed-citation><mixed-citation xml:lang="en">ChoyC.H. Aggregation and size attributes analysis of unadsorbed and adjuvantadsorbed antigens using a multispectral imaging flow cytometer platform / C.H.Choy[et al] // Journal of pharmaceutical sciences. 2021.</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Deng L.Contrasting life strategies of viruses that infect photo- and heterotrophic bacteria, as revealed by viral tagging/ L. Deng[et al] // mBio 2019. Vol. 3, №6.</mixed-citation><mixed-citation xml:lang="en">Deng L.Contrasting life strategies of viruses that infect photo- and heterotrophic bacteria, as revealed by viral tagging/ L. Deng[et al] // mBio 2019. Vol. 3, №6.</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Durdik M. Imaging flow cytometry as a sensitive tool to detect low-dose-induced DNA damage by analyzing 53BP1 and γH2AX foci in human lymphocytes / M. Durdik[et al] // Cytometry. Part A: the journal of the International Society for Analytical Cytology. 2013. Vol. 87, №12. P.1070–1078.</mixed-citation><mixed-citation xml:lang="en">Durdik M. Imaging flow cytometry as a sensitive tool to detect low-dose-induced DNA damage by analyzing 53BP1 and γH2AX foci in human lymphocytes / M. Durdik[et al] // Cytometry. Part A: the journal of the International Society for Analytical Cytology. 2013. Vol. 87, №12. P.1070–1078.</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Fertrin K . Y. Sickle cell imaging flow cytometry assay (SIFCA) / K.Y. Kleber [et al] // Methods in molecular biology. 2016. Vol. 1389. P.279–292.</mixed-citation><mixed-citation xml:lang="en">Fertrin K . Y. Sickle cell imaging flow cytometry assay (SIFCA) / K.Y. Kleber [et al] // Methods in molecular biology. 2016. Vol. 1389. P.279–292.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
